pcr fragment purification kit (TaKaRa)
TaKaRa is a verified supplier 
TaKaRa manufactures this product 
96
Structured Review
TaKaRa
pcr fragment purification kit
Pcr Fragment Purification Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 13579 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+fragment+purification+kit/PCR+Amplification+Kit/pmc12626645-56-36-41
Average 96 stars, based on 13579 article reviews
Pcr Fragment Purification Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 13579 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+fragment+purification+kit/PCR+Amplification+Kit/pmc12626645-56-36-41
Average 96 stars, based on 13579 article reviews
pcr fragment purification kit - by Bioz Stars,
2026-09
96/100 stars
Images
Related Articles
Polymerase Chain Reaction:Article Title: Sucrose-based biofloc system promotes the enrichment of specific gut bacterial taxa to enhance disease suppression in shrimp (Penaeus vannamei) Article Snippet: Sucrose-based biofloc system could facilitate the growth and fitness of shrimp (Penaeus vannamei) by regulating the gut bacterial community.. However, little is known about the specific taxa enriched by sucrose-based biofloc system that could improve shrimp health.. Here, we investigated the influences of sucrose-based biofloc system on the diversity, structure, and function of the shrimp gut bacterial community. Article Title: Bacterial dynamics and biotic sources in the developing swimming crab embryos Article Snippet: The microbial community associated with aquatic animals begins to assemble from the embryonic stage and plays a vital role in their growth and survival.. However, the microbial community of the swimming crab Portunus trituberculatus during its embryonic period has not yet been studied.. In this study, we investigated the embryonic bacterial community (EBC) of this species across the developmental cycle to elucidate its longitudinal dynamics and biotic sources. Article Title: Divergent Response of Host‐Specific Driven Gut Microbial Stability in Freshwater Gastropods to Cyanobacterial Blooms Article Snippet: .. The PCR amplification program was set as follows: 95°C for 3 min, 95°C for 30 s, 50°C for 30 s, and 72°C for 45 s, with the final extension required at 72°C for 10 min. A Article Title: Effects of carbon source addition strategies on water quality, growth performance, and microbial community in shrimp BFT aquaculture systems Article Snippet: The current study evaluated the effect of a novel carbohydrate addition strategy that establishes a mixed biofloc technology aquaculture mode in which heterotrophic bacteria dominate in the early stage and autotrophic bacteria play a role in the latter stage.. The new strategy is divided into three phases: In the early rearing stage, a high C/N ratio (≥15:1) is created by daily input feed and a carbon source to promote the formation of heterotrophic bioflocs; when the ammonia nitrogen concentration is reduced to 1 mg/L, the input C/N ratio is kept at 10:1; stop adding carbon source once there is a clear decrease trend in the nitrite nitrogen.. Fourteen water quality indicators, shrimp growth, and some economic data were selected to evaluate the feasibility of the new strategy at the macro level. Article Title: Biogeographic shifts in the microbial co-occurrence network features of three domains across complex environmental gradients in subtropical coastal waters Article Snippet: .. An equimolar amount of PCR product for each sample was mixed into one pooled sample for each primer set and purified using the Article Title: Multidimensional comparisons of the V4 and V9 regions of the 18S rRNA gene in characterizing microeukaryotic community across complex environmental gradients of coastal waters. Article Snippet: .. PCR amplification for the V4 region was performed under the following conditions: denaturation at 95 ◦C for 3 min, followed by 32 cycles of denaturation at 95 ◦C for 30 s, annealing 55 ◦C for 30 s, and extension 72 ◦C for 1 min, ending with a final extension at 72 ◦C for 10 min. PCR amplification for the V9 region was performed under the following conditions: denaturation at 95 ◦C for 3 min, followed by 32 cycles of denaturation at 95 ◦C for 30 s, annealing 55 ◦C for 30 s, and extension 72 ◦C for 45 s, ending with a final extension at 72 ◦C for 10 min. Triplicate amplicons were mixed, purified using a Article Title: A Comparative Study on Gut Microbiota and Metabolomics in Atlantic Salmon Salmo salar at Different Growth Stages Article Snippet: Atlantic salmon (Salmo salar) represents the primary species in aquaculture.. The gut microbiota plays a crucial role in nutrient processing and protection against pathogenic bacteria.. Nonetheless, the composition and functionality of the gut microbiota in Salmo salar at different growth stages remain largely unexplored. Article Title: Acute Salinity Stress Disrupts Gut Microbiota Homeostasis and Reduces Network Connectivity and Cooperation in Razor Clam Sinonovacula constricta. Article Snippet: Accumulating evidence demonstrates that it is of great importance to maintain a stable and functional gut microbial community for host’s growth and health.. However, gut microenvironment is constantly affected by diverse environmental factors.. Salinity can cause stress, including hypersaline or hyposaline stress to aquatic species, thereby affecting their growth conditions. Purification:Article Title: Sucrose-based biofloc system promotes the enrichment of specific gut bacterial taxa to enhance disease suppression in shrimp (Penaeus vannamei) Article Snippet: Sucrose-based biofloc system could facilitate the growth and fitness of shrimp (Penaeus vannamei) by regulating the gut bacterial community.. However, little is known about the specific taxa enriched by sucrose-based biofloc system that could improve shrimp health.. Here, we investigated the influences of sucrose-based biofloc system on the diversity, structure, and function of the shrimp gut bacterial community. Article Title: Bacterial dynamics and biotic sources in the developing swimming crab embryos Article Snippet: The microbial community associated with aquatic animals begins to assemble from the embryonic stage and plays a vital role in their growth and survival.. However, the microbial community of the swimming crab Portunus trituberculatus during its embryonic period has not yet been studied.. In this study, we investigated the embryonic bacterial community (EBC) of this species across the developmental cycle to elucidate its longitudinal dynamics and biotic sources. Article Title: Divergent Response of Host‐Specific Driven Gut Microbial Stability in Freshwater Gastropods to Cyanobacterial Blooms Article Snippet: .. The PCR amplification program was set as follows: 95°C for 3 min, 95°C for 30 s, 50°C for 30 s, and 72°C for 45 s, with the final extension required at 72°C for 10 min. A Article Title: Effects of carbon source addition strategies on water quality, growth performance, and microbial community in shrimp BFT aquaculture systems Article Snippet: The current study evaluated the effect of a novel carbohydrate addition strategy that establishes a mixed biofloc technology aquaculture mode in which heterotrophic bacteria dominate in the early stage and autotrophic bacteria play a role in the latter stage.. The new strategy is divided into three phases: In the early rearing stage, a high C/N ratio (≥15:1) is created by daily input feed and a carbon source to promote the formation of heterotrophic bioflocs; when the ammonia nitrogen concentration is reduced to 1 mg/L, the input C/N ratio is kept at 10:1; stop adding carbon source once there is a clear decrease trend in the nitrite nitrogen.. Fourteen water quality indicators, shrimp growth, and some economic data were selected to evaluate the feasibility of the new strategy at the macro level. Article Title: Biogeographic shifts in the microbial co-occurrence network features of three domains across complex environmental gradients in subtropical coastal waters Article Snippet: .. An equimolar amount of PCR product for each sample was mixed into one pooled sample for each primer set and purified using the Article Title: Multidimensional comparisons of the V4 and V9 regions of the 18S rRNA gene in characterizing microeukaryotic community across complex environmental gradients of coastal waters. Article Snippet: .. PCR amplification for the V4 region was performed under the following conditions: denaturation at 95 ◦C for 3 min, followed by 32 cycles of denaturation at 95 ◦C for 30 s, annealing 55 ◦C for 30 s, and extension 72 ◦C for 1 min, ending with a final extension at 72 ◦C for 10 min. PCR amplification for the V9 region was performed under the following conditions: denaturation at 95 ◦C for 3 min, followed by 32 cycles of denaturation at 95 ◦C for 30 s, annealing 55 ◦C for 30 s, and extension 72 ◦C for 45 s, ending with a final extension at 72 ◦C for 10 min. Triplicate amplicons were mixed, purified using a Article Title: A Comparative Study on Gut Microbiota and Metabolomics in Atlantic Salmon Salmo salar at Different Growth Stages Article Snippet: Atlantic salmon (Salmo salar) represents the primary species in aquaculture.. The gut microbiota plays a crucial role in nutrient processing and protection against pathogenic bacteria.. Nonetheless, the composition and functionality of the gut microbiota in Salmo salar at different growth stages remain largely unexplored. Article Title: Acute Salinity Stress Disrupts Gut Microbiota Homeostasis and Reduces Network Connectivity and Cooperation in Razor Clam Sinonovacula constricta. Article Snippet: Accumulating evidence demonstrates that it is of great importance to maintain a stable and functional gut microbial community for host’s growth and health.. However, gut microenvironment is constantly affected by diverse environmental factors.. Salinity can cause stress, including hypersaline or hyposaline stress to aquatic species, thereby affecting their growth conditions. Amplification:Article Title: Divergent Response of Host‐Specific Driven Gut Microbial Stability in Freshwater Gastropods to Cyanobacterial Blooms Article Snippet: .. The PCR amplification program was set as follows: 95°C for 3 min, 95°C for 30 s, 50°C for 30 s, and 72°C for 45 s, with the final extension required at 72°C for 10 min. A Article Title: Effects of carbon source addition strategies on water quality, growth performance, and microbial community in shrimp BFT aquaculture systems Article Snippet: The current study evaluated the effect of a novel carbohydrate addition strategy that establishes a mixed biofloc technology aquaculture mode in which heterotrophic bacteria dominate in the early stage and autotrophic bacteria play a role in the latter stage.. The new strategy is divided into three phases: In the early rearing stage, a high C/N ratio (≥15:1) is created by daily input feed and a carbon source to promote the formation of heterotrophic bioflocs; when the ammonia nitrogen concentration is reduced to 1 mg/L, the input C/N ratio is kept at 10:1; stop adding carbon source once there is a clear decrease trend in the nitrite nitrogen.. Fourteen water quality indicators, shrimp growth, and some economic data were selected to evaluate the feasibility of the new strategy at the macro level. Article Title: Multidimensional comparisons of the V4 and V9 regions of the 18S rRNA gene in characterizing microeukaryotic community across complex environmental gradients of coastal waters. Article Snippet: .. PCR amplification for the V4 region was performed under the following conditions: denaturation at 95 ◦C for 3 min, followed by 32 cycles of denaturation at 95 ◦C for 30 s, annealing 55 ◦C for 30 s, and extension 72 ◦C for 1 min, ending with a final extension at 72 ◦C for 10 min. PCR amplification for the V9 region was performed under the following conditions: denaturation at 95 ◦C for 3 min, followed by 32 cycles of denaturation at 95 ◦C for 30 s, annealing 55 ◦C for 30 s, and extension 72 ◦C for 45 s, ending with a final extension at 72 ◦C for 10 min. Triplicate amplicons were mixed, purified using a Article Title: A Comparative Study on Gut Microbiota and Metabolomics in Atlantic Salmon Salmo salar at Different Growth Stages Article Snippet: Atlantic salmon (Salmo salar) represents the primary species in aquaculture.. The gut microbiota plays a crucial role in nutrient processing and protection against pathogenic bacteria.. Nonetheless, the composition and functionality of the gut microbiota in Salmo salar at different growth stages remain largely unexplored. Agarose Gel Electrophoresis:Article Title: Effects of carbon source addition strategies on water quality, growth performance, and microbial community in shrimp BFT aquaculture systems Article Snippet: The current study evaluated the effect of a novel carbohydrate addition strategy that establishes a mixed biofloc technology aquaculture mode in which heterotrophic bacteria dominate in the early stage and autotrophic bacteria play a role in the latter stage.. The new strategy is divided into three phases: In the early rearing stage, a high C/N ratio (≥15:1) is created by daily input feed and a carbon source to promote the formation of heterotrophic bioflocs; when the ammonia nitrogen concentration is reduced to 1 mg/L, the input C/N ratio is kept at 10:1; stop adding carbon source once there is a clear decrease trend in the nitrite nitrogen.. Fourteen water quality indicators, shrimp growth, and some economic data were selected to evaluate the feasibility of the new strategy at the macro level. Article Title: A Comparative Study on Gut Microbiota and Metabolomics in Atlantic Salmon Salmo salar at Different Growth Stages Article Snippet: Atlantic salmon (Salmo salar) represents the primary species in aquaculture.. The gut microbiota plays a crucial role in nutrient processing and protection against pathogenic bacteria.. Nonetheless, the composition and functionality of the gut microbiota in Salmo salar at different growth stages remain largely unexplored. |